Here are some guides, templates, software and drivers that you might find useful in your western blotting ventures.
- Protocol
- General procedures of performing a western blot
- Quantitation guide
- Instructions for quantitating your blots with ImageJ
- Organising and processing quantitated data
- Instructions for processing and tidying up quantitated data on a spreadsheet before performing statistical analyses
- BCA calculation template
- Template for calculating original protein concentrations in each sample, and normalisation of protein concentrations across all your samples
- Buffer recipes
- Simplified, one-page bench instructions for making up western blot buffers
- Refrigerator temperature log
- Template for tracking refrigerator temps so that your primary antibodies don't go bad
Software and drivers
I keep these drivers up on my site as a means to preserve them, seeing as they are only available on CDs that come with the original machine. In the event that those CDs are destroyed or degraded (due to cosmic rays or whatnot), at least I have them backed up here. So sue me.
- Fujifilm LAS-3000 (Version 2.2)
- Drivers for the imaging system that we use in our labs
- MultiGauge (Version 3.0)
- Software for post-processing of raw images obtained from the imaging system
Serial number: 381470